Multivariate analysis of fluorescence and source identification of dissolved organic matter in Jiaozhou Bay, China

Hierarchical clustering analysis and principal component analysis (PCA) methods were used to assess the similarities and dissimilarities of the entire Excitation-emission matrix spectroscopy (EEMs) data sets of samples collected from Jiaozhou Bay, China. The results demonstrate that multivariate analysis facilitates the complex data treatment and spectral sorting processes, and also enhances the probability to reveal otherwise hidden information concerning the chemical characteristics of the dissolved organic matter (DOM). The distribution of different water samples as revealed by multivariate results has been used to track the movement of DOM material in the study area, and the interpretation is supported by the results obtained from the numerical simulation model of substance tracing technique, which show that the substance discharged by Haibo River can be distributed in Jiaozhou Bay.     

Ex situ echo sounder target strengths of ice krill Euphausia crystallorophias

Ice krill is the keystone species in the neritic ecosystem in the Southern Ocean,where it replaces the more oceanic Antarctic krill. It is essential to understand the variation of target strength(TS in d B re 1 m 2) with the different body size to accurately estimate ice krill stocks. However,there is comparatively little knowledge of the acoustic backscatter of ice krill. The TS of individual,formalin-preserved,tethered ice krill was measured in a freshwater test tank at 38,120,and 200 k Hz with a calibrated split-beam echo sounder system. Mean TS was obtained from 21 individual ice krill with a broad range of body lengths(L :13–36 mm). The length( L,mm) to wet weight( W; mg) relationship for ice krill was W =0.001 218×103 × L 3.53( R 2= 0.96). The mean TS-to-length relationship were TS3 8 k Hz =-177.4+57log 10( L),( R 2 = 0.86); TS 120 k Hz =-129.9+31.56 log 10( L),( R 2 =0.87); and TS 200 k Hz =-117.6+24.66log1 0( L),( R 2 =0.84). Empirical estimates of the relationship between the TS and body length of ice krill were established at 38,120,and 200 k Hz and compared with predictions obtained from both the linear regression model of Greene et al.(1991) and the Stochastic Distorted Wave Born Approximation(SDWBA) model. This result might be applied to improve acoustic detection and density estimation of ice krill in the Southern Ocean. Further comparative studies are needed with in situ target strength including various body lengths of ice krill.     

Species delimitation in the green algal genus Codium (Bryopsidales) from Korea using DNA barcoding

Codium, one of the largest marine green algal genera, is difficult to delimit species boundary accurately based on morphological identification only. DNA barcoding is a powerful tool for discriminating species of seaweeds. The plastid elongation factor TU(tuf A) is considered as maker to perform DNA barcoding of green algal species than rbc L gene due to universality and rapid evolution rate. We conducted DNA barcoding application to Codium specimens from the Jeju Island, Korea to overcome the limit of morphological identification and to confirm the species diversity. As a result of applying tuf A marker, we newly generated fifty-five tuf A barcodes to resolve eight species. Tuf A marker exhibited 6.1%–21.8% interspecific divergences, wider than the gap of rbc L exon 1,3.5%–11.5%. Molecular analysis of rbc L exon 1 sequences of Codium revealed eight distinct species like tuf A analysis separated in five phylogenetic groups. DNA barcoding of the genus Codium using tuf A marker is more helpful to overcome the limit of morphological identification, and this is more potential to reveal cryptic species and to resolve the relationships among subspecies than rbc L analysis alone. The complement of tuf A barcoding and rbc L analyses including morphology for the genus Codium in the northwestern Pacific will give much more reliable achievement for discovering species diversity and resolving the phylogenetic relationships.     

MIL 03443, a dunite from asteroid 4 Vesta: Evidence for its classification and cumulate origin

Abstract– The absence of dunite (>90 vol% olivine) in the howardite, eucrite, and diogenite (HED) meteorite suite, when viewed with respect to spectroscopic and petrologic evidence for olivine on Vesta, is problematic. Herein, we present petrologic, geochemical, and isotopic evidence confirming that Miller Range (MIL) 03443, containing 91 vol% olivine, should be classified with the HED clan rather than with mesosiderites. Similarities in olivine and pyroxene FeO/MnO ratios, mineral compositions, and unusual mineral inclusions between MIL 03443 and the diogenites support their formation on a common parent body. This hypothesis is bolstered by oxygen isotopic and bulk geochemical data. Beyond evidence for its reclassification, we present observations and interpretations that MIL 03443 is probably a crustal cumulate rock like the diogenites, rather than a sample of the Vestan mantle.     

The influence of thermohaline fronts on chlorophyll a concentrations during spring and summer in the southeastern Yellow Sea

A spatial and temporal variation in physiochemical parameters in the southeastern Yellow Sea(YS) is investigated in the spring and summer of 2009 to 2011.Nutrient show a strong negative relationship with chlorophyll a(Chl a) concentration in spring,and the subsurface chlorophyll a maxima(SCM) layer was associated with the nitracline in summer.In summer,the SCM was usually found within or above the pycnocline and at the depths of shoals from the open sea to the coastal sea due to tidal and/or topographical fronts in the southernmost study area.High Chl a concentrations were found in the central southern YS,where the YS cold water layer expanded under the pycnocline and encountered water masses during spring and summer.After a typhoon in the summer of 2011,Chl a concentration increased,especially in the central southern YS,where cold waters occurred below the pycnocline.The results suggest that the development of thermohaline fronts may play an important role in the growth and accumulation of phytoplankton biomass in the upper layer of the southeastern YS during spring and summer.     

A New Fossil Termite(Isoptera,Stolotermitidae,Stolotermes)from the Early Miocene of Otago,New Zealand

<正>The forewing of a termite from Early Miocene lake sediments in Otago,southern New Zealand is figured and described.It exhibits the generic characters of the damp-wood termite Stolotermes Hagen,but differs from forewings of the known species in size and venation pattern and is described as Stolotermes kupe sp.nov.S.kupe represents the first confident record of fossil Stolotermitidae and extends the fossil record of the family back to the Early Miocene.It also is the first direct evidence of fossil Isoptera from New Zealand,though silicified termite faecal pellets,referable to Kalotermes brauni,have been previously described.S.kupe indicates that Stolotermitidae has been present in the Australasian region since at least the Early Miocene.     

GIS and SBF for estimating groundwater recharge of a mountainous basin in the Wu River watershed,Taiwan

The temporal and spatial distributions of precipitation are extremely uneven; so, careful management of water resources in Taiwan is crucial. The long-term overexploitation of groundwater resources poses a challenge to water resource management in Taiwan. However, assessing groundwater resources in mountainous basins is challenging due to limited information. In this study, a geographic information system (GIS) and stable base-flow (SBF) techniques were used to assess the characteristics of groundwater recharge considering the Wu River watershed in central Taiwan as a study area. First, a GIS approach was used to integrate five contributing factors: lithology, land cover/land use, lineaments, drainage, and slope. The weights of factors contributing to the groundwater recharge were obtained from aerial photos, geological maps, a land use database, and field verification. Second, the SBF was used to estimate the groundwater recharge in a mountainous basin scale. The concept of the SBF technique was to separate the base-flow from the total streamflow discharge in order to obtain a measure of groundwater recharge. The SBF technique has the advantage of integrating groundwater recharge across an entire basin without complex hydro-geologic modelling and detailed knowledge of the soil characteristics. In this study, our approach for estimating recharge provides not only an estimate of how much water becomes groundwater, but also explains the characteristics of a potential groundwater recharge zone.     

Geochemistry and Origin of Layers in Single Manganese Nodule from the Philippine Sea and Manganese Nodules from Offshore Minami-Torishima,Western Pacific

Layers from one manganese nodule dredged from the Philippine Sea(16°56'N, 129°48'E; water depth, 5700 m) and 45 bulk nodules from offshore Minami-Torishima Island, Japan(23°3'N, 153°22'E; water depth, 1200 m) were analyzed chemically and their origin is discussed based on geochemical constraints. In general, Cu, Ni, Zn and Mo tend to increase with increasing Mn content, while Co, Pb, Ba, V, Sc, Th, and the rare earth elements(REEs) show less variation with increasing Mn content. Nodule 42 H from the Philippine Sea has an average Mn/Fe ratio close to 1 and shows a positive Ce anomaly, suggesting a predominant hydrogenous origin. Profiles of 230Th230 ex and Thex/232 Th ratios in the outer ~0.3 mm of nodule 42 H indicate a steady growth rate of ~1.7 mm/Myr. Nodule E30 from offshore Minami-Torishima is characterized by lower Mn, Fe, Mn/Fe(0.53) and Mo/V(0.2) ratios but higher P and Cu/Ni(0.31) ratio relative to other nodules from that area. The Ce content of E30 is unusually low(82 ppm) when compared with other nodules from the area and it is the only nodule analyzed with a negative Ce anomaly(-0.64). Based on the geochemical data we suggest that most nodules from offshore Minami-Torishima are primarily of hydrogenous origin except E30, which is dominated by hydrothermal input, and E45, which has about a 35% hydrothermal contribution.